Western blot analysis of lysates from HeLa cells, using Amyloid beta A4 (Phospho-Thr743/668) Antibody. The lane on the right is blocked with the phospho peptide.
Immunogen
The antiserum was produced against synthesized peptide derived from human Amyloid beta A4 around the phosphorylation site of Thr743/668.
Immunogen Range
711-760
Modification Sites
H: T743 M: T743 R: T743
MW
86 kDa
NCBI Gene Symbol
APP
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Specificity
Amyloid beta A4 (Phospho-Thr743/668) Antibody detects endogenous levels of Amyloid beta A4 only when phosphorylated at Thr743/668.